Service Cat. # BZ17001
When it comes to Molecular Cloning, who doesn't need Superpower?
Using the most advanced cloning technologies, our Molecular Biologists can insert any sequence (or almost...) into any vector (or almost...) at any location (or almost...). Our directional cloning approach is restriction site-independent, which allows great flexibility:
Insertion of your gene(s) at any specified location within the destination vector(s) (independent of nuclease-recognition sites).
Modification of up to 6 bp (substitution/insertion/deletion) at both cloning sites on the insert(s) and/or vector(s) to insert a Kozak sequence, an additional stop codon, a linker, change the first or last residues,...
Examples of 5' cloning site modifications during cloning (destination vector - insert):
...ATCCTGCAACTCCGAGTCGGATTTC... Modification 1 (substitutions)
...ATCCTG------ATGGTCGGATTTC... Modification 2 (deletion)
...ATCCTGCAAGTCCTTGCAATGGTCGGATTTC... Modification 3 (insertion)
Material provided by customers:
Gene to clone: plasmid containing the gene (>500 ng) or sequence to synthesize (Gene Synthesis).
Destination vector (>500 ng).
DNA sequence (as text) of the empty destination vector (or flanking sequences) and gene of interest.
Miniprep of the cloned plasmid (Maxiprep or higher amounts available - optional).
DNA sequence data file covering the inserted gene.
Typical molecular cloning turnaround time is 8-12 days for inserts of up to 1.5 kb.
The actual processing time is typically influenced by the size of the destination vector(s) and/or the insert(s) and the complexity of their sequence.