Service Cat. # BZ17006

Protein Scale Up

 

We offer several scale up options for large scale protein preparation in bacteria (E. coli) and yeast (P. pastoris). We have prepared multiple types of recombinant proteins in the past, yielding from milligram to gram amounts of protein using common purification approaches.

 

We recommend to first optimize the expression and purification conditions using our screening platform prior to scale up.

The purified proteins are typically shipped in dry ice in the recommended storage buffer but we can also aliquot and lyophilize your final product.

 

Ready to accelerate your research?    Request a quote.

Turnaround times are based on the amount of initial material (culture volume) but most protein expression/purification scale ups are performed in just 4-5 days.

1)   Culture and First Purification Step:

Prices of protein expression and purification scale up services in E. coli is function of the number of construct/protein prepared and of the amount of starting material (volume of culture).

Below prices include: Protein expression + IMAC protein purification + Dialysis in storage buffer. Prices are total price, not price per construct. Protein scale up from rich media such as TB are charged an extra 15%.

1 Construct

$589

$599

$678

$857

$1,049

$1,228

$1,399

Volume of Culture for Each Construct (in L)

0.5

1

2

4

6

8

10

2 Constructs

$727

$739

$928

$1,347

$1,669

$1,959

$2,199

4 Constructs

$888

$947

$1,379

$2,149

$2,649

$3,099

$3,499

Prices in USD

2)   Second/Third Purification Step:

Prices are based on number of run performed or number of protein construct purified simultaneously.

Prices are total price, not price per run/construct.

Number of Run or Construct

 

1

2

3

4

Number of Run or Construct

 

1

2

3

4

1 mL Column

$289

$337

$383

$428

5 mL Column

$357

$458

$557

$652

Gradient-Based Elution Column (such as ion exchanger)

XK26 Column

$369

$472

$571

$668

Size-Exclusion Column (Superdex-75 or -200)

XK16 Column

$289

$367

$459

$548

Prices in USD

If you have more constructs to scale up, need higher starting material (culture volume) or have any question, contact us at info@biozilla.bio.

 Material provided by customers:

 

You can send us the sequence of your protein(s) that we could gene synthesize and subclone into our proprietary expression plasmids. You can also send us your plasmids.

For plasmid submission: Send us >0.5 ug DNA, dry or liquid form following our Sample Submission instructions.   Select EASYSHIPPING for a fast and affordable sample submission: Just place your samples in an envelope, print the shipping documents that you'll receive and relax, we even set up the pick up for you.

Deliverables:

  

  • Entire amount of purified protein.

  • Protein expression and purification scale up report.

To place an order, please send us an email with the following info. You'll receive a correponding quote shortly after.

 

  • Name and sequence of the protein(s) to screen.

  • If you submit a plasmid, name of the vector, antibiotic resistance and type of induction.

  • Number of conditions/variables to screen. Let us know if you need specific conditions to test and if you expect the protein(s) to be relatively difficult or easy to express.

Your samples can be stored in our safe and secured storage facility for further uses or as back up for as low as $0.06/month.

Like all our products, your protein samples will be carbon free certified.

Fact: A small lab of 1000 sq.ft uses in average 200 kWh of electricity per day (www.goodcampus.org), emiting ~250 lbs of CO2. This generates as much greenhouse gases as a car driving from Seattle, WA to Miami, FL  every day.

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