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Service Cat. # BZ17002
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Site-Directed Mutagenesis
"Mutate" is a 2nd generation Site-Directed Mutagenesis service which offers the following advantages over traditional approaches:
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More To Mutate: Substitutions or insertions of up to 24 consecutive bp; fragment deletion of unlimited size. Simultaneous modifications at two or three different sites. Amazing possibilities (examples below)!
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Highest Fidelity Reaction: Novel PCR-based approach using the highest fidelity polymerase identified to date (10 to 20 times higher fidelity than the Pfu used in QuickChange) makes unwanted second-site errors a thing of the past.
![Site directed mutagensis](https://static.wixstatic.com/media/85df7b_37d4268e06b44461a476b8ea2cebcfc0.png/v1/fill/w_237,h_186,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/85df7b_37d4268e06b44461a476b8ea2cebcfc0.png)
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Affordable Service: as low as $119 for a single reaction and $79 for bulk orders!
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Rapid: Mutated plasmid typically delivered 8-12 days after reception of the original plasmid.
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Relax: This is a service, not a kit... Send us your plasmids and the desired mutations and you'll receive a Miniprep of the mutated plasmids and the corresponding DNA sequencing data (Maxiprep or larger amounts available at extra cost).
Examples of Mutations which can be performed in a single reaction:
...ATGCTGGTCCTAAGCCATTCAGCGTCCATCTTAGCTCACCGTAAGCTC... Original vector
...ATGCTGGTCCTAAGCCATTCAGCGTCCATCTCCACTCACCGTAAGCTC... Mutation 1 (substitutions)
...ATGCTGGCGCGTATCCCGAGCTAGGATTTCGTAGCTCACCGTAAGCTC... Mutation 2 (substitutions)
...ATG------------------------------------------CTC... Mutation 3 (deletion)
...ATGCTGGTCCTAAGCCATTCAGCGTCCATCTTAGTACCGATCGCTCACCGTAAGCTC... Mutation 4 (insertion)
Material provided by customers:
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Original plasmid to mutate (>500 ng).
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DNA sequence (as text) of the original plasmid and the mutated plasmid. Please use the template file downloadable below on this page to provide all the necessary information.
Deliverables:
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Miniprep of the mutated plasmid (Maxiprep or higher amounts available - optional).
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DNA sequence data file surrounding the mutation (extended coverage available upon request).
Mutant Libraries:
Our site-directed mutagenesis approach offers great flexibility and can be adapted to the preparation of various types of mutant libraries such as:
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Single Point and Scanning Point libraries: Substitution to any of the 19 common amino acids at single or multiple positions. Delivered as isolated or pool of variants.
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Randomized and Degenerated Libraries: Any modification, within 24 bp. Delivered as a pool of variants.
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Please request a quote for pricing.
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Additional price (based on plasmid size and number of bp to mutate):
Plasmid Size
< 6 kb
6 - 8 kb
8 - 10 kb
> 10 kb
bp to Mutate
< 6
7 - 15
16 - 24
25 - 500
Mutate Reaction (Unit)
1 - 5
6 - 10
11 - 15
16 - 20
21 - 30
>31
Prices in US Dollar.
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Your DNA samples can be stored in our safe and secured storage facility for further uses or as back up for as low as $0.06/month.
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Like all our products, your DNA mutant will be carbon free certified.
Fact: A small lab of 1000 sq.ft uses in average 200 kWh of electricity per day (www.goodcampus.org), emiting ~250 lbs of CO2. This generates as much greenhouse gases as a car driving from Seattle, WA to Miami, FL every day.