PetitePrep Service vs Midiprep Kit
Comparative: Biozilla's Service vs Commercial Kit
Three plasmids were prepared in parallel using either the Hipure Midiprep Kit from Thermo Fisher Scientific or Biozilla's Petiteprep service.
With a typical yield at or close to 0.5 mg DNA, the Biozilla's PetitePrep service provides more than five times higher plasmid DNA quantity than a Midiprep Kit.
The PetitePrep service produces also plasmid DNA of higher purity as judged by spectrophotometric ratios (260/280 = 1.8 - 1.9 & 260/230 > 2.2 for pure plasmids) and agarose gel electrophoresis (below).
All tested plasmids are high copy number. Plasmid 1, 2 and 3 are respectively 5.5 kb, 5.7 kb and 5.6 kb. Plasmid preparations using the HiPure Midiprep kit (Thermo Fisher Scientific) has been performed following the manufacturer's recommended procedure.
Conclusion - The PetitePrep service from Biozilla provides higher DNA quantity and better DNA purity than a Midiprep kit.
Why Biozilla's plasmid preps outperform the commercial kits and other CRO's services?
--- Our secret is no secret ! ---
We use larger bacterial culture as starting material compared to the kit.
Volume of processed culture (in mL) per purification column.
We wash our resin more thoroughly than the kits.
Volume of Wash Buffer (in mL) applied to each column prior to plasmid DNA elution.
Our plasmid DNA preparation process takes more time than the kits.
Time of plasmid DNA purification (in hour), bacterial culture excluded.
Conclusion - Our secret is no secret ! We use larger culture volume, wash our columns more thoroughly and as a result spend more time processing your plasmids. These are some of the reasons Biozilla's plasmid preps produce higher DNA quantity and better DNA quality than the kits and other CROs.